Anticholinesterase refers to an agent that inhibits action of acetylcholinesterase (AChE). AChE is an enzyme that inactivates acetylcholine , a neurotransmitter at the junctions of nerve and muscle cells (neuromuscular junctions) that regulates the delivery of messages from neurons to muscle fibers . By inhibiting the activity of AChE, such medications allow high levels of acetylcholine to accumulate, potentially enabling repeated stimulation of nerves at neuromuscular junctions.

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